Application of bioethical aspects in the management of modified MS media waste in tissue culture laboratories

Abstract

The development of biotechnology through plant tissue culture techniques provides significant benefits in producing superior, disease-free seedlings quickly. However, the use of modified Murashige and Skoog (MS) media also produces laboratory waste that has the potential to pollute the environment if not managed properly. This study aims to examine the management of tissue culture media waste, identify potential risks, and analyze the application of bioethical principles in its management. This study uses a descriptive qualitative approach with data collection techniques through observation and documentation in the Tissue Culture Laboratory. The results show that contaminated culture media waste is still disposed of directly without undergoing a sterilization process and waste separation according to standards. This condition has the potential to cause negative impacts such as the spread of microorganisms, environmental pollution due to chemical content, and the risk of health problems. From a bioethical perspective, this practice does not fully implement the principles of non-maleficence, beneficence, and responsibility. Therefore, the implementation of waste management in accordance with bioethical principles through sterilization, waste separation, and sustainable management is needed to support safe and responsible laboratory practices.